METHODS FOR ADDING ADAPTERS TO NUCLEIC ACIDS AND COMPOSITIONS FOR PRACTICING THE SAME

摘要

Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3′ hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.

主权项

1. A method comprising:
combining: a template ribonucleic acid (RNA); a template switch oligonucleotide comprising a 3′ hybridization domain and a sequencing platform adapter construct; a polymerase; and dNTPs; in a reaction mixture under conditions sufficient to produce a product nucleic acid comprising the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid comprising a region polymerized from the dNTPs by the polymerase.