发明名称 |
CELL DIFFERENTIATION ASSAY METHOD, CELL ISOLATION METHOD, METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS, AND METHOD FOR PRODUCING DIFFERENTIATED CELLS |
摘要 |
Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc;” and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc.” |
申请公布号 |
US2015111218(A1) |
申请公布日期 |
2015.04.23 |
申请号 |
US201314381116 |
申请日期 |
2013.02.27 |
申请人 |
National Institute of Advanced Industrial Science and Technology ;Wako Pure Chemical Industries, Ltd. |
发明人 |
Tateno Hiroaki;Hirabayashi Jun;Asashima Makoto;Ito Yuzuru;Onuma Yasuko;Warashina Masaki;Fukuda Masakazu |
分类号 |
G01N33/569 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
1. A method for determining differentiation of a cell comprising:
a step of contacting a test cell with a probe comprising a protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell: (A) a protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc;” and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc.” |
地址 |
Tokyo JP |