| 摘要 |
Provided is a method for the detection of a protein, such as haemoglobin or a metalloprotein, in a test sample, such as faecal sample, the method comprising the steps of: (i) exposing the sample to a surface having a binding moiety and permitting the binding moiety to specifically immobilise the protein in the sample; (ii) permitting the immobilised protein to catalyse a reaction of a first reagent with a second reagent; and (iii) detecting, such as electrochemically detecting, the reaction of the first reagent with the second reagent. The binding moiety may be provided on the surface of an electrode. Also provided is a method for detecting an analyte in a sample, which analyte is a binding partner for a protein, the method comprising the steps of: (i) providing a protein that is immobilised, such as specifically immobilised, to a surface; (ii) exposing the immobilised protein to the analyte in the sample, thereby to bind the analyte to the immobilised protein; (iii) exposing the immobilised protein to a first reagent and a second reagent; and (iv) detecting, such as electrochemically detecting, the reaction of first reagent with the second reagent, wherein the immobilised protein is a catalyst for the reaction of the first reagent with the second reagent in the absence of the analyte, and the binding of the analyte to the immobilised protein alters the catalytic activity of the protein for the reaction of the first reagent with the second reagent. The binding moiety can be an antibody. The first reagent can be tetramethylbenzidine and the second reagent can be hydrogen peroxide. |
