Laminous Vascular Constructs Combining Cell Sheet Engineering And Electrospinning Technologies

摘要

Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.

主权项

1. A method of producing a tissue engineered vascular construct, comprising:
forming an electrospun biocompatible matrix in a tubular configuration; culturing a cell population comprising smooth muscle cells to form a cell sheet and applying the cell sheet to the outside of the matrix to form a vascular construct.