发明名称 |
Competitive chemiluminescent assay for cyclic nucleotide monophosphates |
摘要 |
<p>A competitive assay for the concentration of a cyclic nucleotide such as cyclic adenosine monophosphate combines, in a reaction chamber provided with a capture antibody, an antibody for the cAMP or other cyclic nucleotide, the sample to be assayed and a conjugate of cAMP and alkaline phosphatase. The mixture is incubated and washed, and after washing, a 1,2-dioxetane which is caused to decompose when contacted by the alkaline phosphatase of said conjugate is added. The light emission caused by decomposition is measured, with the strength of the signal being inversely related to the concentration of the cyclic nucleotide. Optionally, an enhancement agent in the form of a polymeric onium salt may be added, to further enhance the light emission.</p> |
申请公布号 |
EP2213311(B1) |
申请公布日期 |
2015.04.22 |
申请号 |
EP20100158650 |
申请日期 |
2000.05.02 |
申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
BRONSTEIN, IRENA;CHIULLI, ANTHONY C.;PALMER, MICHELLE A. J.;VOYTA, JOHN C. |
分类号 |
A61K51/00;C09K3/00;C12Q1/06;C12Q1/42;G01N21/78;G01N33/53;G01N33/563;G01N33/569 |
主分类号 |
A61K51/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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