摘要 |
The disclosed flow cytometer (40) includes; 1. a laser diode ("LD") based optical subsystem (50) for impinging a beam of light upon particles passing through a viewing zone; 2. a composite microscope objective (60) for gathering and imaging light scattered from or fluoresced by particles passing through the viewing zone; 3. a fluidic subsystem (70) for supplying a liquid sheath flow to the viewing zone; 4. a peristaltic pump (80) for injecting into the liquid sheath flow a liquid sample flow carrying particles that passes together with the liquid sheath flow through the viewing zone; 5. a multimode optical fiber (852) that receives scattered and fluoresced light from the viewing zone that the composite microscope objective (60) gathers and images; and 6. a wavelength division multiplexer (90) for optically separating into color bands light received via the optical fiber (852). |