Apparatus for and method of automated processing of biological samples

摘要

Provided herein is a bioprocessing device, bioprocessing card, and fluidics cartridge for performing automated bioprocessing of a sample. The bioprocessing card may include a plurality of pipette tips; and at least one pump in fluid communication with the plurality of pipette tips. In some embodiments, the pumps and the pipette tips are in fluid communication through a processing channel which may be a microscale channel. Also provided herein is an automated bioprocessing device comprising: at least one bioprocessing card; at least one fluidic cartridge; and an automated control system configured to control automated bioprocessing of a sample. Further provided herein are methods of use of the device, card, and cartridge.

主权项

1. A system for sample bioprocessing, comprising:
a. a fluidics cartridge that includes a first well and a second well, wherein the first well holds a fluid sample that includes one or more magnetic particles; b. a bioprocessing card that includes a first pipette tip, a second pipette tip, and a pump that are in fluid communication along a processing channel and that is placed in proximity to the fluidics cartridge so that the first pipette tip is in fluid communication with the first well and the second pipette tip is in fluid communication with the second well wherein the processing channel is a mesoscale channel or a microscale channel, the first pipette tip and second pipette tip include individual valves; c. a manifold that is in physical communication with the bioprocessing card; d. a magnetic assembly including at least one electromagnet or permanent magnet that is placed in proximity to the fluidics cartridge; e. a memory that includes instructions for a sample preparation protocol; and f. a processor that is in electronic communication with the manifold, the magnetic assembly, and the memory, that reads the instructions from the memory, and that according to the instructions
i. activates the magnetic assembly to apply a magnetic field to the first well so that at least one magnetic particle of the one or more magnetic particles of the fluid sample is attracted andii. signals the manifold to apply pressure and/or vacuum to the bioprocessing card activating the fluid valves of the first pipette tip, the second pipette tip, and the pump to move a portion of the fluid sample from the first well though the processing channel, to the second well producing a bioprocessed sample in the first well; g. a housing containing the fluidics cartridge, the bioprocessing card, the manifold, the magnetic assembly, the memory and the processor, wherein at least one vent is located on a portion of the housing configured to provide heat dispersion.