Method for mass-producing antifreeze protein derived from polar yeast

摘要

The present invention relates to a method for mass-producing an antifreeze protein derived from a polar yeast, and more particularly, to a method for mass-producing an antifreeze protein derived from Leucosporidium sp., which is the polar yeast, for synthesizing a recombinant polynucleotide by optimizing and altering a gene, which codes the antifreeze protein derived from the polar yeast, for a yeast expression system, and for expressing same using the yeast expression system.

主权项

1. A method for mass-producing an antifreeze protein derived from arctic yeast or a culture medium containing the same, comprising the steps of:
(1) synthesizing a polynucleotide comprising the nucleotide sequence of SEQ. ID. NO: 1; (2) introducing the polynucleotide of step (1) into a Pichia pastoris-derived expression vector using an alcohol oxidase promoter (AOX1) to prepare a recombinant expression vector; (3) culturing a yeast transformant transformed with the recombinant expression vector; and (4) obtaining a culture medium from step (3).