Dipeptides to enhance yield and viability from cell cultures

摘要

The present invention relates to the culture of animal cells in serum-free culture medium. The present invention provides particular dipeptides that can improve recombinant protein production and cell viability in such cultures, especially in the absence of peptones.

主权项

1. A method of culturing Chinese hamster ovary (CHO) cells that have been recombinantly engineered to express a protein, the method comprising growing the CHO cells in a serum-free medium during a growth phase, and growing the CHO cells in a serum-free defined production medium during a production phase, wherein during the production phase the serum-free medium is supplemented with at least one dipeptide selected from Tyr-His, Tyr-Lys, His-Gly, and Ala-His, and wherein the titer of the protein is improved in the presence of the dipeptide or dipeptides as compared to the absence of the dipeptide or dipeptides.