Methods for manufacturing molecular arrays

摘要

The methods of the present invention provide methods for manufacturing a master substrate and methods for manufacturing replica arrays from the master substrate. The methods may be used, for example, directly to manufacture or “print” peptide arrays from a DNA array; however, the methods are applicable to a wide range of manufacturing applications for use any time multiple copies of an array needs to be printed.

主权项

1. A method of manufacturing an array of products on a replica array comprising:
delivering a continuous volume of a liquid reagent or reaction mix on the surface of a master substrate by laminar flow; providing partitioned reaction volumes of the liquid reagent or reaction mix on the master substrate, wherein the reaction volumes are partitioned by surface energetic barriers provided by adjacent hydrophobic regions and hydrophilic regions on the master substrate; generating products by one or more enzymatic processes in the partitioned reaction volumes of the liquid reagent or reaction mix; and immobilizing the products from the partitioned reaction volumes from the master substrate on capture moieties disposed on a replica array that is in contact with the partitioned reaction volumes, wherein partitioning of the reaction volumes prevents diffusion of products between them.