Broad spectrum vaccine against typhoidal and non-typhoidal <i>Salmonella </i>disease

摘要

The present invention is drawn to multivalent Salmonella enterica serovar conjugate vaccines comprising conjugates of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi, S. Paratyphi A and optionally S. Paratyphi B, wherein the conjugates comprise a hapten antigen and a carrier antigen, wherein at least one of the hapten antigens or carrier antigens is characteristic of the Salmonella enterica serovar. The present invention also provides Salmonella enterica serovar reagent strains to produce the multivalent conjugate vaccines and attenuated Salmonella enterica serovars for use as vaccines.

主权项

1. A method of inducing an immune response, comprising administering to a subject in need thereof an immunologically-effective amount of a composition comprising
1) a conjugate comprising a phase 1 flagella protein from the Salmonella enterica serovar S. Typhimurium wherein the phase 1 flagella protein is FliC or FliCI411A and a core-O polysaccharide, wherein the core-O polysaccharide is from a Salmonella enterica serovar selected from the group consisting of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi and S. Paratyphi A and combinations thereof; 2) a conjugate comprising a phase 1 flagella protein from the Salmonella enterica serovar S. Enteritidis wherein the phase 1 flagella protein is FliC or FliCI411A and a core-O polysaccharide, wherein the core-O polysaccharide is from a Salmonella enterica serovar selected from the group consisting of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi and S. Paratyphi A and combinations thereof; 3) a conjugate comprising a phase 1 flagella protein from the Salmonella enterica serovar S. Choleraesuis wherein the phase 1 flagella protein is FliC or FliCI411A and a core-O polysaccharide, wherein the core-O polysaccharide is from a Salmonella enterica serovar selected from the group consisting of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi and S. Paratyphi A and combinations thereof; 4) a conjugate comprising a phase 1 flagella protein from the Salmonella enterica serovar S. Typhi wherein the phase 1 flagella protein is FliC or FliCI411A and a core-O polysaccharide, wherein the core-O polysaccharide is from a Salmonella enterica serovar selected from the group consisting of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi and S. Paratyphi A and combinations thereof; and 5) a conjugate comprising a phase 1 flagella protein from the Salmonella enterica serovar S. Paratyphi A wherein the phase 1 flagella protein is FliC or FliCI411A and a core-O polysaccharide, wherein the core-O polysaccharide is from a Salmonella enterica serovar selected from the group consisting of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi and S. Paratyphi A and combinations thereof.