Differentiation of human embryonic stem cells

摘要

The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides a method to increase the expression of markers associated with the pancreatic endocrine lineage.

主权项

1. A method of increasing the expression of markers associated with pancreatic endocrine cells comprising treating human pancreatic endocrine cells with medium comprising a sufficient amount of a TGF-β receptor agonist for about one to about five days to cause an increase in expression of markers associated with the pancreatic endocrine cells compared to pancreatic endocrine cells not treated with the TGF-β receptor agonist, wherein the method comprises an increase in expression of PDX-1, NKX6.1, and PAX4.