ω-Aminocarboxylic acids, ω-aminocarboxylic acid esters, or recombinant cells which produce lactams thereof

摘要

The present invention relates to a cell, which has been genetically modified relative to its wild type, so that in comparison with its wild type it is able to produce more ω-aminocarboxylic acids, more ω-aminocarboxylic acid esters or more lactams derived from ω-aminocarboxylic acids, starting from carboxylic acids or carboxylic acid esters. Furthermore, the present invention relates to a method for the production of a genetically modified cell, the cells obtainable by this method, a method for the production of ω-aminocarboxylic acids, of ω-aminocarboxylic acid esters or of lactams derived from ω-aminocarboxylic acids, the ω-aminocarboxylic acids, ω-aminocarboxylic acid esters or lactams derived from ω-aminocarboxylic acids obtainable by this method, a method for the production of polyamides based on ω-aminocarboxylic acids or based on lactams and the polyamides obtainable by this method.

主权项

1. An isolated recombinant cell, which has been genetically modified relative to its wild type so that, in comparison to the wild type, the recombinant cell is able to produce more ω-aminocarboxylic acid, ω-aminocarboxylic acid esters, or more lactams derived from ω-aminocarboxylic acid, starting from carboxylic acids, or carboxylic acid esters, wherein the isolated recombinant cell comprises:
(i) a first nucleic acid encoding a heterologous enzyme EI, wherein the enzyme EI catalyzes the conversion of carboxylic acids or carboxylic acid esters to the corresponding ω-aminocarboxylic acid or ω-aminocarboxylic acid esters, and wherein the enzyme EI comprises an alkane monooxygenase encoded by the alkBGT gene from Psudomonas putida GPo1 comprising the amino acid sequences set forth in SEQ ID NOS: 50, 51, and 52, and a cytochrome P450 monooxygenase from Candida tropicalis; and (ii) a second nucleic acid encoding at least one of a heterologous enzyme EII and a heterologous enzyme EIII, wherein the enzyme EII catalyzes the conversion of ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters to the corresponding ω-oxocarboxylic acids or ω-oxocarboxylic acid esters, wherein the enzyme EII is selected from the group consisting of an alkane monooxygenase, alcohol dehydrogenase, and alcohol oxidase, and wherein the enzyme EIII catalyzes the conversion of ω-oxocarboxylic acids or ω-oxocarboxylic acid esters to the corresponding ω-aminocarboxylic acids or ω-aminocarboxylic acid esters, wherein the enzyme EIII is a ω-transaminase, wherein the expression of the first and second nucleic acids results in overexpression of the enzyme EI and overexpression of at least one of enzyme EII and enzyme EIII and wherein said expression increases the production of ω-aminocarboxylic acid, ω-aminocarboxylic acid esters, or lactams derived from ω-aminocarboxylic acid compared to the wild type cell.