Syntese af 2 ', 3'- dideoxynukleosider til automatiseret DNA-syntese og PYROPHOSPHOROLYSIS AKTIVERET POLYMERISERING

摘要

Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.