METHOD AND SYSTEM FOR POLYPEPTIDE PURIFICATION

摘要

The present invention provides a method and automated system for the purification of polypeptides including the direct filtration of solutions containing the polypeptides after purification.

主权项

1. A Fast Protein Liquid Chromatography (FPLC) based method for purifying a polypeptide comprising the following steps:
a) applying a liquid solution containing the polypeptide to a chromatographic means containing a stationary phase; b) separating the polypeptide from other molecules contained in the liquid solution by passing the liquid solution through the stationary phase of the chromatographic means; c) recovering the polypeptide from the chromatographic means in a flow-through or an eluate; and d) sterilizing the flow-through or eluate containing the recovered polypeptide by filtration;wherein said sterile filtration is in-line sterile filtration which is carried out directly after chromatography.