Process and Device for Separating Biological Particles Contained in a Fluid by Means of Filtration

摘要

The invention relates to a method of separating biological particles from the liquid containing same for purification, analysis and optionally diagnostic purposes. The inventive method comprises at least one step involving vertical filtration through a filter having a porosity that is adapted to the type of biological particles to be separated, such that said particles are retained by the filter. The invention is characterised in that: (i) the method involves the use of a filter comprising at least one basic filtration zone, whereby each basic filtration zone has a limited surface area; and (ii) the surface area of each basic filtration zone and the number of basic filtration zones are selected as a function of the type of liquid to be filtered, the type of biological particles to be separated and the volume of liquid to be filtered.

主权项

1. Process for seeking rare characteristic cells in a biological fluid for diagnosis purposes, comprising:
a) a step of treatment of the biological fluid for lysis of the cells that are of no interest and for anticoagulation, b) a step of vertical filtration of filtering a biological fluid through a filter consisting of a membrane comprising pores calibrated to a size of between 3 μm and 100 μm and the density of which is between 3×103 and 5×106 per cm2, said filter comprising elementary filtration areas, each elementary filtration area having a surface equal to that of a disk with a diameter of between 0.6 cm and 3 cm, and wherein the number of elementary filtration areas is so that the ratio of the volume of the fluid to be filtered and the sum of the surfaces of the plurality of elementary filtration areas is between 0.14 ml/cm2 and 40 ml/cm2; and, c) a step of analysis of the cells retained on the filtration areas.