| 摘要 |
A fluorimetric system, apparatus and method are disclosed to measure an analyte concentration in a sample. A sensor reagent housing is also disclosed which comprises a channel and a porous medium having an analysis chemistry reagent, such as a nucleic acid analysis chemistry reagent. The apparatus and system are portable for field use, with an apparatus housing having a size adapted to fit into a user's hand. An apparatus also includes a sensing chamber and cover, a user interface, a light source; a photomultiplier tube, an amplifier, an A/D converter, a memory, and a processor. In various embodiments, the processor performs a data fitting, determines a sample reaction rate parameter, and determines the analyte concentration from a comparison of the sample reaction rate parameter with stored calibration data. The processor may also generate the calibration data and site-specific offset factors, and modify the calibration data using an offset factor. |
| 主权项 |
1. A fluorimetric system to determine an analyte concentration in a sample, the system comprising:
a fluorimetric apparatus; and a sensor reagent housing coupleable to the fluorimetric apparatus, the sensor reagent housing comprising a sensor housing and a porous substrate, the porous substrate having an analysis chemistry reagent; and the sensor housing comprising a sensor cap and a sensor base, wherein the sensor cap comprises a first channel and a first interlocking region, the first channel having a first diameter at the first interlocking region; and wherein the sensor base comprises: a second interlocking region to mate with the first interlocking region; a second channel holding the porous substrate and in fluid communication with the first channel, the second channel having a second diameter greater than the first diameter; and a third channel coupled to and in fluid communication with the second channel, the third channel having a third diameter smaller than both the first diameter and the second diameter. |
