发明名称 |
Multiphase nucleic acid amplification |
摘要 |
<p>Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.</p> |
申请公布号 |
AU2013308647(A1) |
申请公布日期 |
2015.04.16 |
申请号 |
AU20130308647 |
申请日期 |
2013.08.30 |
申请人 |
GEN-PROBE INCORPORATED |
发明人 |
NELSON, NORMAN C.;ARNOLD JR., LYLE J.;DAI, LIZHONG;PHELPS, STEVEN S.;CHELLISERRY, JIJUMON |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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