| 发明名称 |
METHOD FOR ANALYSIS OF COMPOUND-BINDING ABILITY OF PROTEIN |
| 摘要 |
A method for analyzing a binding ability of protein to a compound, involves fractionating first and second groups of proteins into plural fractions using a carrier having the compound immobilized thereon; combining fractions, analyzing the combined fractions with mass spectrometry; based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between peaks derived from a protein in each of the groups of fractions; and comparing degrees of the binding ability of the plural kinds of proteins to the compound. |
| 申请公布号 |
US2015105292(A1) |
申请公布日期 |
2015.04.16 |
| 申请号 |
US201414517504 |
申请日期 |
2014.10.17 |
| 申请人 |
EISAI R&D MANAGEMENT CO., |
发明人 |
Oda Yoshiya;Katayama Hiroyuki |
| 分类号 |
G01N33/68;G01N33/58;G01N33/543 |
主分类号 |
G01N33/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for analyzing a binding ability of protein to a compound, comprising:
(a) fractionating a first group of proteins into plural fractions using a carrier having the compound immobilized thereon; (b) adding a certain amount of a second group of proteins to each of the fractions obtained in step (a); (c) analyzing the fractions obtained in step (b) with mass spectrometry; and (d) based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between a peak derived from a protein in the fraction obtained in step (a) and a peak derived from a protein in the second group of proteins, and comparing degrees of the binding ability of the plural kinds of proteins to the compound. |
| 地址 |
Tokyo JP |
