| 主权项 |
1. Method for the targeted modulation of the gene expression, comprising the steps:
(i) Provision of a target nucleic acid sequence to be expressed, (ii) Modification of the target nucleic acid sequence, in which the number of CpG dinucleotides present in the target nucleic acid sequence is raised using the degeneracy of the genetic code to increase the gene expression, or is lowered to reduce the gene expression, (iii) Cloning of the thereby modified target nucleic acid sequence with a modified number of the CpG dinucleotides in a suitable expression vector in operative coupling with a suitable transcription control sequence, (iv) Expression of the modified target nucleic acid sequence in a suitable expression system. |
