发明名称 |
METHODS AND COMPOSITIONS FOR DETERMINATION OF VECTOR BACKBONE IN A NUCLEIC ACID SAMPLE |
摘要 |
<p>The invention provides methods and compositions for detecting and/or quantifying vector backbone in a nucleic acid preparation comprising a polynucleotide of interest using amplification assays that amplify a junction located between the polynucleotide of interest and the vector backbone, under conditions whereby amplification can occur, wherein the junction comprises a recognition site for a nuclease, and detecting the absence of an amplification product, whereby the absence of the amplification product indicates low or no vector backbone and/or quantifying the amount of amplification product to determine the amount of vector backbone in the nucleic acid preparation.</p> |
申请公布号 |
EP2859122(A2) |
申请公布日期 |
2015.04.15 |
申请号 |
EP20130804003 |
申请日期 |
2013.06.10 |
申请人 |
SYNGENTA PARTICIPATIONS AG |
发明人 |
HE, CHENGKUN;FAN, CHUNYANG;KE, JOHN;ZHONG, HENG;RUSSELL, DOUG |
分类号 |
C12Q1/68;C12N15/82;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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