Assay with internal calibration

摘要

Provided herein are kits and methods for assays with internal calibration.

主权项

1. A method of internally calibrating an assay, comprising:
a) contacting a capture analyte-binding molecule with a predetermined concentration of tracer analyte labeled with a first label and a predetermined concentration of detection analyte-binding molecule labeled with a second label, wherein the capture analyte-binding molecule and the detection analyte-binding molecule concurrently bind to the tracer analyte; b) measuring the signal intensities obtained of the first label (I10) and of the second label (I20); c) determining a correction factor (F) as the ratio of the intensity of the second label (I20) to the intensity of the first label (I10); and d) internally calibrating the assay by dividing the predetermined concentration of tracer analyte by the correction factor (F).