Separation of rebaudioside A from stevia glycosides using chromatography

摘要

Disclosed are methods for the chromatographic separation of rebaudioside A from stevioside in glycoside solutions that are derived from stevia. The chromatographic separation may be an adsorb/desorb type of chromatographic separation or a fractionation type of chromatographic separation.

主权项

1. A method of separating rebaudioside A from stevioside using chromatography, the method comprising the steps of:
(a) providing a glycoside solution comprising rebaudioside A; stevioside; and a solvent; (b) providing an adsorbent comprising a polystyrene divinylbenzene support functionalized with tertiary amines, quaternary amines, or both tertiary and quaternary amines; and (c) contacting the adsorbent with the glycoside solution in order to separate at least a portion of the rebaudioside A from the stevioside thereby resulting in the formation of a purified rebaudioside A solution.