Apparatus and method for suppression of background noise in microscopy imaging

摘要

An apparatus and method for imaging a section of a medium is disclosed. The apparatus or method receives and returns light from the section and from sites adjacent to the section. A microscope using this apparatus or method can be telecentric in pinhole space at the detection end of the system.

主权项

1. An apparatus for imaging a section of a medium which receives and returns light from the section and from sites adjacent to the section, the apparatus comprising:
an optical system for directing light in beams of different polarization in the medium along an optical axis and returning light from the medium, the optical system comprising:
a pinhole lens assembly configured to receive the returned light, the pinhole lens assembly having a focal length, and wherein the focal length is configured such that a first focal point of the pinhole lens assembly is located substantially at a pupil of the optical system such that the pinhole assembly is telecentric; and a detector configured to receive the returned light from the pinhole lens assembly and produce an electrical signal in response to the received light.