Pregnancy-associated plasma protein-A2 (PAPP-A2) polynucleotides

摘要

The present invention provides pregnancy associated plasma protein A2 (PAPP-A2), its nucleotide and amino acid sequences, antisense molecules to the nucleotide sequences which encode PAPP-A2, expression vectors for the production of purified PAPP-A2, antibodies capable of binding specifically to PAPP-A2, hybridization probes or oligonucleotides for the detection of PAPP-A2-encoding nucleotide sequences, genetically engineered host cells for the expression of PAPP-A2, and methods for screening for pathologies in pregnant and non-pregnant patients. Methods for screening for altered focal proliferation states in pregnant and/or non-pregnant patients, which include detecting levels of PAPP-A2, are also described.

主权项

1. A cDNA that encodes a polypeptide that
(a) consists of amino acid residues 234-1791 of SEQ ID NO: 2 (mature pregnancy associated plasma protein A2 (PAPP-A2)); or (b) is at least 95% sequence identical to the polypeptide of (a), and differs from the polypeptide of (a) solely by
(i) deletion of 1-10 amino acid residues from, or addition of 1-10 residues to, the amino terminal, and/or(ii) deletion of 1-10 amino acid residues from, or addition of 1-10 residues to, the carboxy terminal, and/or(iii) one or more conservative substitutions; wherein said polypeptide has a proteolytic activity against Insulin Like Growth Factor Binding Protein 5 (IGFBP-5).