Device and method for determining the concentration of fluorophores in a sample

摘要

A device (10) and a method for analyzing a sample (16) containing fluorophores use a light source (12) emitting light (λex) onto the sample (16), and onto a fluorescence standard (14). The fluorophores of the sample (16), given an immission of light of a first wavelength (λex1), have a first excitation efficiency and, given an immission of light of a second wavelength (λex2), have a second excitation efficiency. The fluorescence standard (14), given the same immissions of light, has a third excitation efficiency and, a fourth excitation efficiency. An optical element (20) which is arranged between the light source (12) and the sample (16) and/or (12) the fluorescence standard (14) adapts, due to its optical property, a first difference between the first excitation efficiency and the second excitation efficiency and a second difference between the third excitation efficiency and the fourth excitation efficiency to each other.

主权项

1. A device for determining the concentration of fluorophores in a sample,
with a light source (12) for emitting light onto the sample (16) and for emitting light onto a fluorescence standard (14, 46), wherein the fluorophores of the sample (16), given an immission of light of a first wavelength (λex1) have a first excitation efficiency and, given an immission of light of a second wavelength (λex2), have a second excitation efficiency, (λex2), wherein the fluorophores of the fluorescence standard (14, 46), given an immission of light of the first wavelength (λex1) have a third excitation efficiency and, given an immission of light of the second wavelength (λex2) onto the fluorescence standard (14, 46), have a fourth excitation efficiency, with an optical element (20, 42) which is arranged between the light source (12) and the sample (16) and/or between the light source (12) and the fluorescence standard (14, 46) and/or as an optically effective layer on the fluorescence standard (14) and which, due to its optical property, adapts a first difference between the first excitation efficiency and the second excitation efficiency and a second difference between the third excitation efficiency and the fourth excitation efficiency to each other.