Methods and compositions for limiting viability of a modified host cell outside of designated process conditions

摘要

The invention provides methods and compositions for inhibiting proliferation of a modified host cell outside of a designated process condition. Compositions and methods for providing a host cell having reduced viability when exposed to natural conditions external to a controlled environment are disclosed.

主权项

1. A method for altering viability of a Synechococcus cell, comprising the steps of:
a. providing a non-nutrient ambient process condition for use with said cell, wherein said process condition is present in a controlled environment and absent outside said controlled environment; and b. producing a genetically modified cell, wherein the genetically modified cell comprises one or more genetic modifications, wherein said one or more genetic modifications comprises an inactivated phr gene.