发明名称 |
Recombinant microorganism having butanol production capacity and butanol production method |
摘要 |
This invention relates to a recombinant microorganism having improved butanol production capacity and butanol production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and a group of genes involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA are introduced into a host microorganism. |
申请公布号 |
US9005953(B2) |
申请公布日期 |
2015.04.14 |
申请号 |
US201012775048 |
申请日期 |
2010.05.06 |
申请人 |
Toyota Jidosha Kabushiki Kaisha |
发明人 |
Muramatsu Masayoshi;Obata Shusei;Yoneda Satoshi;Sugimura Masahiro;Kuzuyama Tomohisa |
分类号 |
C12N1/21;C12P7/16;C12N9/02;C12N9/10 |
主分类号 |
C12N1/21 |
代理机构 |
Sughrue Mion, PLLC |
代理人 |
Sughrue Mion, PLLC |
主权项 |
1. A recombinant microorganism, comprising an acetoacetyl-CoA synthase gene encoding a protein having the amino acid sequence of SEQ ID NO: 1, and a group of genes from Clostridium acetobutvlicum that are involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA, wherein the genes are introduced into a host microorganism, wherein the group of genes involved in butanol biosynthesis consists of the β-hydroxybutyryl-CoA dehydrogenase gene, the 3-hydroxybutyryl-CoA dehydratase gene, the butyryl-CoA dehydrogenase gene, the butyraldehyde dehydrogenase gene, and the butanol dehydrogenase gene, wherein the acetoacetyl-CoA synthase gene is a gene from an actinomycete of the genus Streptomyces, and wherein the host microorganism is Escherichia coli. |
地址 |
Aichi JP |