| 摘要 |
<p>TAPS is a new low cost media for fungal and bacterial plant pathogen and is therefore a replacement for potato-dextrose-agar media used for fungal cultures and nutrient-sucrose-agar media used for bacterial cultures. The ingredients of TAPS medium i.e. topico granules powder (10g), agar agar powder (10gm) and sugar (20gm) is added into 250 ml of potato broth (prepared from 250gm potato) and the final volume is made to 1 liter with distilled water, before sterilization of the medium. The ingredient of TAPS medium i.e. tapioca granule's powder, agar agar powder and sugar in 1:1:2 proportion can be mixed together in large quantities to stored in plastic jars. This ingredient mixture can be stored for a considerable longer period for 6 month and above. 40 gm of this ingredient is added to 250 ml of potato broth, whenever the TAPS medium has to be prepared and the final volume is made to 1 liter. The medium is useful for culturing both fungal and bacterial plant pathogen. The constituent of TAPS medium can be stored in plastic jars in laboratory as a single ingredient. The composite ingredient is easy to handle and facilitate quick preparation of the TAPS medium. TAPS as a medium and its ingredients composite is not reported earlier. The ingredient of TAPS as a single composite has commercial value in the research/practical laboratories and therefore needs patent.</p> |
