METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON CFP10-DBD HYBRID PROTEIN AND DEXTRANE; pCFP10-DBD RECOMBINANT PLASMIDE; Escherichia coli [pREP4, pCFP10-DBD] STRAIN; CFP10-DBD CHIMERIC PROTEIN AND THEIR APPLICATION

摘要

FIELD: biotechnologies.SUBSTANCE: invention represents pCFP10-DBD recombinant plasmide consisting of an artificial bacterial operon of a chimeric protein including a promoter region of early promoter of T5 bacteriophage, a chimeric protein gene consisting of a sequence of protein antigene CFP10 from Mycobacterium tuberculosis, which is merged with a sequence of a dextrane-binding domain (DBD) of dextrane sucrase Leuconostoc citreum KM20, and a transcription terminator, a bacterial operon of beta-lactamase and a bacterial initiation section of replication of ColE1 type. Besides, strain Escherichia coli - a producer of CFP10-DBD chimeric protein is presented. The invention describes a method of immobilisation, concentration and cleaning of the obtained protein on cellulose. The invention describes an immunogenic composition containing CFP10-DBD recombinant protein, which is aimed at induction of immunity against tuberculous infection.EFFECT: invention enlarges the range of devices used for treatment of tuberculosis.4 cl, 6 dwg, 1 tbl, 4 ex