NOVEL INORGANIC METALLIC NANO-PARTICULATE GENE CARRIER FOR EFFECTIVE BIOTRANSFORMATION AND COMPOSITION THEREOF

摘要

The present invention relates to the HMG CoA reductase gene encapsulated inorganic metallic nanoparticle and its application in plant biotransformation. More particularly, invention relates to calcium phosphate nanoparticle preparation actively conjugated/encapsulated with HMG Co-A reductase genes and its application in plant biotransformation process. The invention further relates to process of entrapping genetic material in nanoparticles of calcium phosphate of size below 100nm diameter to form non-viral carrier which can be usedfor the delivery of genes in plant of Cichoriumintybus L. The invention provides for the use of micellar solubilisation technique as a method of synthesis of HMG CoA reductase gene encapsulated calcium phosphate nanoparticle. The proposed method of biotransformation process in plant Cichoriumintybus L. mediated by calcium phosphate nanoparticle encapsulated with HMG CoA reductase gene results in biotransformation and survival rate upto 30% ( in less than 10min) and 50% respectively with enhanced biosynthesis of secondry metabolite.