FARNESENE-SYNTHASE

摘要

FIELD: chemistry.SUBSTANCE: invention relates to the field of biotechnology. Claimed are: a separated from the tomato polynucleotide, corresponding to SEQ ID NO:1, presented in the description, or a polynucleotide, which has, at least, a 70% identity with the nucleotide sequence SEQ ID NO:1, or their fragment, which code a polypeptide with the activity of farnesene-synthase, corresponding to SEQ ID NO:2, presented in the description, or having, at least, a 70% identity with an amino acid sequence, corresponding to SEQ ID NO:2, or their fragment. Genetic constructions, containing the said polynucleotide, where genetic constructions represent a transformation vector and an expression vector are described. Described is the transformation vector, which contains the polynucleotide which is hybridised with a polynucleotide, coding the said polypeptide. A host cell for expression farnesene-synthase, which contains a genetic construction, selected from the said ones, is described. Claimed is a method of obtaining alpha-farnesene and/or beta-farnesene, including stages of the said host cell cultivation with obtaining the increased activity of alpha-farnesene synthase and/or activity of beta-farnesene-synthase and optionally obtaining a cell with farnesyldiphosphate; and the separation of the obtained alpha-farnesene and/or beta-farnesene. Described is a method of modulating the production of alpha-farnesene and beta-farnesene by a plant, which includes an increase or reduction of expression of the said farnesene-synthase, where the said increase or reduction is achieved by means of genetic modification to change the expression of polynucleotide, coding the said polypeptide. Claimed is the application of the polynucleotide in accordance with the claimed invention for coding a molecular (DNA) marker, bound with the said nucleotide sequence.EFFECT: invention makes it possible to obtain alpha-farnesene and beta-farnesene in the host cell.16 cl, 8 dwg, 1 tbl, 1 ex