SOYBEAN TRANSFORMATION METHOD

摘要

The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.

主权项

1. A method of identifying shoots created from soybean germline transformants, the method comprising:
a. transforming a population of cells of a soybean plant with a transgene, wherein the population of transformed cells comprises transformed germline cells and transformed non-germline cells; b. regenerating shoots from the population of transformed cells; c. isolating the shoots produced by the population of transformed cells; d. subjecting the isolated regenerated shoots to a selective rooting medium, wherein (i) the subjected isolated regenerated shoots produced by the transformed germline cells create viable roots, and (ii) the subjected isolated regenerated shoots produced by the transformed non-germline cells do not create viable roots; and e. identifying the shoots created from soybean germline transformants by detecting whether or not the shoot creates viable roots.