New PCR sequencing method and use thereof in HLA genotyping

摘要

The present invention provides a PCR sequencing method, which comprises the steps of: 1) providing the sample; 2) amplifying; 3) mixing; 4) breaking; 5) sequencing and 6) splicing. The present invention also provides the primer tags used in the method, as well as the use of the method in genotyping, especially in HLA analysis. The present invention also provides PCR primers for HLA-A/B, HLA-C and HLA-DQB1.