| 发明名称 |
NADP-DEPENDENT ALANINE DEHYDROGENASE |
| 摘要 |
The present invention relates to a polypeptide comprising the amino acid sequence of the Bacillus subtilis alanine dehydrogenase or a variant thereof, where Leu197 or an amino acid which is located at a homologous position in the amino acid sequence is exchanged for an amino acid with a positively charged side chain, to a nucleic acid molecule encoding such a polypeptide, and to a process for the production of alanine or a compound generated with consumption of alanine, comprising the step of reacting pyruvate with ammonium and NADPH to give alanine by bringing the pyruvate into contact with the polypeptide according to the invention or with the cell according to the invention. |
| 申请公布号 |
US2015099282(A1) |
申请公布日期 |
2015.04.09 |
| 申请号 |
US201314395666 |
申请日期 |
2013.04.16 |
| 申请人 |
Haas Thomas;Pfeffer Jan Christoph;Skerra Arne;Lerchner Alexandra;Jarasch Alexander |
发明人 |
Haas Thomas;Pfeffer Jan Christoph;Skerra Arne;Lerchner Alexandra;Jarasch Alexander |
| 分类号 |
C12P13/06;C12N9/06 |
主分类号 |
C12P13/06 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A cell comprising
a polypeptide comprising the amino acid sequence of the Bacillus subtilis alanine dehydrogenase or a variant thereof, where Leu197 or an amino acid located at a homologous position in the amino acid sequence is exchanged for an amino acid with a positively charged side chain, or a nucleic acid molecule comprising a nucleotide sequence encoding the polypeptide. |
| 地址 |
Muenster DE |
