METHODS FOR GENERATING TRANSGENIC PLANTS

摘要

This disclosure provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present disclosure enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette.