| 发明名称 |
Method of Performing Digital PCR |
| 摘要 |
A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample. |
| 申请公布号 |
US2015099644(A1) |
申请公布日期 |
2015.04.09 |
| 申请号 |
US201314395456 |
申请日期 |
2013.04.19 |
| 申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
Chen Caifu;McFarland Casey;Keys David |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of detection of a target nucleic acid comprising:
fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes; subjecting the plurality of sample volumes to conditions for amplification; detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present; counting the number of fractions with an amplified target nucleic acid; and determining the quantity of target nucleic acid in the sample. |
| 地址 |
Carlsbad CA US |
