METHODS AND COMPOSITIONS FOR THE DIAGNOSIS OF SEPSIS USING GAMMA PEPTIDE NUCLEIC ACIDS

摘要

The present disclosure provides for composition of γPNA probes. Additionally, the present disclosure provide for methods and kits using γPNA probes for the prognosis of sepsis. In some embodiments, wherein the γPNA capture probes and γPNA reporter probes comprise one or more functional moiety selected from the group consisting of: a binding molecule, a spacer group, a linker group, a hydrophobicity-changing group, a charge-inducing group, and a structural charge-inducing group.

主权项

1. A method for diagnosing sepsis comprising:
(a) contacting a plurality of γPNA capture probes to genomic material in a clinical sample obtained from a subject suspected of having sepsis, wherein the γPNA capture probes comprise at least one sequence from one or more of the groups of probes selected from the group consisting of: SEQ ID NOS: 1-18, SEQ ID NOS: 19-22, SEQ ID NOS: 23-28, SEQ ID NOS: 29-34, SEQ ID NOS: 35-38, SEQ ID NOS: 39-57, SEQ ID NOS: 58-72, SEQ ID NOS: 73-91, SEQ ID NOS: 92-94, SEQ ID NOS: 95-97, SEQ ID NOS: 98-110, SEQ ID NOS: 111-113, SEQ ID NOS: 114-117, SEQ ID NOS: 118-119, SEQ ID NOS: 120-121, SEQ ID NOS: 122-153, SEQ ID NOS: 154-166, SEQ ID NOS: 167-190, SEQ ID NOS: 191-193, SEQ ID NOS: 194-196, SEQ ID NOS: 197-211, SEQ ID NOS: 212-215, SEQ ID NOS: 216-230, complementary sequence thereof, and functional equivalents thereof; (b) heating the γPNA capture probes and the clinical sample; (c) invading a plurality of targeted sepsis-related genomic material by the γPNA capture probes; and (d) detecting a presence of one or more targeted genomic material, wherein detection of the presence of targeted genomic material is indicative of a sepsis infection.