| 摘要 |
Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, the disclosure provides probes and primers that are suitable in dynamic flux amplification procedures. In aspects, the disclosure provides long oligonucleotide probes and primers, as well as triplex forming probes and primers, which function within the narrow Tm ranges used with dynamic flux amplification. However, embodiments are also provided wherein the probes and primers taught herein can be utilized in standard PCR. |
| 主权项 |
1. A nucleic acid system, comprising:
a) a downstream oligonucleotide probe, comprising at least 50 base pairs, having a sequence that is complementary to a first sequence of a target oligonucleotide and that has a cleavable sequence; and b) an upstream oligonucleotide primer, comprising at least 50 base pairs, having a sequence that is complementary to a second sequence of the target oligonucleotide and that has an initial nucleic acid polymerase binding site, wherein the downstream oligonucleotide probe is configured for a polymerase to cleave mononucleotides or small oligonucleotides at its 5′ end, and wherein the Tm of the downstream oligonucleotide probe and the upstream oligonucleotide primer is within about 15° C. of the target nucleic acid Tm. |
