发明名称 |
PROCESS OF DIAGNOSTIC, PROGNOSTIC AND THERAPEUTIC MONITORING OF SOLID TUMORS |
摘要 |
A process of diagnostic, prognostic and therapeutic monitoring of solid tumors, and new biological markers of tumor. |
申请公布号 |
US2015099653(A1) |
申请公布日期 |
2015.04.09 |
申请号 |
US201214354246 |
申请日期 |
2012.10.29 |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ;UNIVERSITE MONTPELLIER 2 SCIENCES ET TECHNIQUES ;INSTITUT CURIE |
发明人 |
Battini Jean-Luc;Decaudin Didier;Massonnet Gerald;Petit Vincent;Sitbon Marc |
分类号 |
G01N33/574;C12N5/071 |
主分类号 |
G01N33/574 |
代理机构 |
|
代理人 |
|
主权项 |
1. Recovery process of single viable cells from a solid tumor comprising two tissue dissociation steps using a non enzymatic dissociation buffer (NEDB) and an enzymatic tissue dissociation, in particular consisting of collagenase III and DNase I to obtain a mixture of isolated dead or viable cells and debris, followed by a cell purification step with a dual density Ficoll™ to eliminate red cells and debris, and thus enrich said mixture in single viable cells. |
地址 |
PARIS FR |