发明名称 PROCESS OF DIAGNOSTIC, PROGNOSTIC AND THERAPEUTIC MONITORING OF SOLID TUMORS
摘要 A process of diagnostic, prognostic and therapeutic monitoring of solid tumors, and new biological markers of tumor.
申请公布号 US2015099653(A1) 申请公布日期 2015.04.09
申请号 US201214354246 申请日期 2012.10.29
申请人 CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ;UNIVERSITE MONTPELLIER 2 SCIENCES ET TECHNIQUES ;INSTITUT CURIE 发明人 Battini Jean-Luc;Decaudin Didier;Massonnet Gerald;Petit Vincent;Sitbon Marc
分类号 G01N33/574;C12N5/071 主分类号 G01N33/574
代理机构 代理人
主权项 1. Recovery process of single viable cells from a solid tumor comprising two tissue dissociation steps using a non enzymatic dissociation buffer (NEDB) and an enzymatic tissue dissociation, in particular consisting of collagenase III and DNase I to obtain a mixture of isolated dead or viable cells and debris, followed by a cell purification step with a dual density Ficoll™ to eliminate red cells and debris, and thus enrich said mixture in single viable cells.
地址 PARIS FR