发明名称 |
METHOD FOR SPECIFIC IDENTIFICATION OF TARGET BIOMOLECULES |
摘要 |
The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other. In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished. |
申请公布号 |
EP2726877(A4) |
申请公布日期 |
2015.04.08 |
申请号 |
EP20120803774 |
申请日期 |
2012.06.27 |
申请人 |
GE HEALTHCARE BIO-SCIENCES AB |
发明人 |
BJÖRKESTEN, LENNART;EDLUND, SOFIA;HAGNER-MCWHIRTER, ÅSA |
分类号 |
G01N33/561;G01N27/447;G01N33/543 |
主分类号 |
G01N33/561 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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