摘要 |
<p>The present invention provides a method and device for achieving identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and to provide a method and device for achieving a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C1, and a second predetermined threshold is C2 (C1 < C2), a colony satisfying C2 ‰¤ C is determined as the undifferentiated colony, a colony satisfying C1 ‰¤ C < C2 is determined as an undefined colony, and a colony satisfying C < C1 is determined as the differentiated colony.</p> |