HIGHLY GLYCOSYLATED LONG-ACTING HUMAN GROWTH HORMONE PROTEIN AND PRODUCTION METHOD FOR SAME

摘要

The present invention relates to a long-acting human growth hormone NexP-hGH protein which is highly glycosylated and highly long acting in vivo, and relates to a production method for same. More specifically, the present invention relates to a specific isoform of long-acting human growth hormone NexP-hGH protein in which human growth hormone is fused with a highly glycosylated alpha-1 antitrypsin mutant whereby long-acting properties in vivo are increased, and relates to a production method for same. Insofar as the sustained human growth hormone production method is concerned, the present invention also relates to a high-purity purification method for NexP-hGH, the method specifically comprising the steps of: (a) carrying out anion-exchange resin chromatography on a biological emulsion comprising NexP-hGH which is a long-acting human growth hormone in which human growth hormone is fused with an alpha-1 antitrypsin mutant; (b) carrying out hydrophobic resin chromatography on the biological emulsion comprising NexP-hGH, or on the eluate produced in step (a); and (c) carrying out affinity chromatography, entailing packing with a resin to which anti-alpha-1 antitrypsin antibody fragments are attached, on the biological emulsion comprising NexP-hGH which is a long-acting human growth hormone, and on the eluate produced in step (a) or step (b).