Methods for obtaining cyclopamine

摘要

The present invention relates to a process for extracting, purifying and isolating cyclopamine from cyclopamine-containing biomass comprising the steps of contacting cyclopamine-containing organic matter with an extractant (e.g., aqueous extractant) capable of extracting cyclopamine, separating the extracted organic matter from the cyclopamine/extract solution, concentrating the cyclopamine/extract solution leaving a concentrated aqueous solution, contacting the concentrated aqueous solution with an organic extractant capable of selectively extracting cyclopamine from the aqueous phase, concentrating the cyclopamine-containing organic phase, providing an enriched extract containing cyclopamine, separating cyclopamine from the enriched extract using column chromatography, providing at least one fraction containing cyclopamine, and crystallizing select chromatography fractions to provide purified cyclopamine.

主权项

1. A process for extracting, purifying and isolating cyclopamine from cyclopamine-containing biomass comprising the steps of:
Mixing the cyclopamine-containing organic matter with an aqueous solvent, which solvent is transparent to microwaves; extracting cyclopamine using microwave-assisted technology to obtain a cyclopamine/extract solution separating the extracted organic matter from the cyclopamine/extract solution; concentrating the cyclopamine/extract solution leaving a concentrated aqueous solution; contacting the concentrated aqueous solution with an organic extractant capable of selectively extracting cyclopamine from the aqueous phase; concentrating the cyclopamine-containing organic phase, providing an enriched extract containing cyclopamine; separating cyclopamine from the enriched extract using column chromatography, providing at least one fraction containing cyclopamine; and crystallizing select chromatography fractions to provide purified cyclopamine.