| 发明名称 |
Method for measuring ATR inhibition mediated increases in DNA damage |
| 摘要 |
The present relates to methods for detecting DNA damage in subjects treated with an ATR inhibitor. More specifically, this invention relates to a method for measuring changes in levels of γH2AX and/or pChk1Ser345 in, e.g., surrogate tissue cells, following ex vivo stimulation with a DNA damaging agent. |
| 申请公布号 |
US8999632(B2) |
申请公布日期 |
2015.04.07 |
| 申请号 |
US201314045373 |
申请日期 |
2013.10.03 |
| 申请人 |
Vertex Pharmaceuticals Incorporated |
发明人 |
Falcon Susanna;Reaper Philip;Pollard John;Curtin Nicola;Middleton Fiona;Chen Tao |
| 分类号 |
A01N1/02;C12Q1/48;G01N33/50;G01N33/68 |
主分类号 |
A01N1/02 |
| 代理机构 |
|
代理人 |
Stewart Rory C. |
| 主权项 |
1. A method for monitoring DNA damage in a subject by measuring accumulation of γH2AX from blood, the method comprising:
a) treating a subject with an ATR inhibitor; b) taking blood samples from the subject at various intervals; c) treating the blood samples with a DNA damaging agent; d) isolating white blood cells from the blood samples; and e) measuring γH2AX levels in the white blood cells by using antibodies specific for γH2AX. |
| 地址 |
Boston MA US |
