Isolation of nucleic acids

摘要

Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.

主权项

1. A method of producing a filtrate from a sample, the method comprising:
a) placing a sample to be filtered into a spin filter, said spin filter comprising
i) a hollow body (1);ii) a bottom end (2); andiii) an opening (3) at a top end of said hollow body (1) opposite the bottom end (2),wherein said hollow body (1) and said bottom end (2) are both composed of the same porous filtering material; and b) centrifuging said spin filter,wherein during said centrifuging, a fraction of said sample passes through said porous filtering material of said spin filter to produce a filtrate.