| 发明名称 | Detection and visualization of the cell cycle in living cells | ||
| 摘要 | The present invention relates to a nucleic acid molecule encoding a polypeptide specifically binding to proliferating cell nuclear antigen (PCNA). The present invention also relates to a vector comprising the nucleic acid molecule of the invention, a host cell comprising the nucleic acid molecule of or the vector of the invention and a method of detecting the amount and/or location of PCNA in living cells, a method of screening for compounds having an effect on the cell cycle. | ||
| 申请公布号 | US8999669(B2) | 申请公布日期 | 2015.04.07 |
| 申请号 | US201013382302 | 申请日期 | 2010.07.06 |
| 申请人 | Ludwig-Maximilians Universitat München | 发明人 | Rothbauer Ulrich;Leonhardt Heinrich;Zolghadr Kourosh;Romer Tina;Schmidthals Katrin |
| 分类号 | C12P21/06;C07K16/18;G01N33/58;G01N33/68 | 主分类号 | C12P21/06 |
| 代理机构 | DT Ward, P.C. | 代理人 | Ward Donna T.;Zhu Heng;DT Ward, P.C. |
| 主权项 | 1. A synthetic nucleic acid molecule encoding a polypeptide which specifically binds to proliferating cell nuclear antigen (PCNA), said nucleic acid molecule comprising a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence deviating from SEQ ID NO: 2 by conservative substitution of 2 or 3 amino acids in position 1 to 28 and 38 to 52 of SEQ ID NO: 2. | ||
| 地址 | Munich DE | ||