发明名称 |
Methods and compositions for producing fatty alcohols |
摘要 |
Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described. |
申请公布号 |
US8999686(B2) |
申请公布日期 |
2015.04.07 |
申请号 |
US200912575430 |
申请日期 |
2009.10.07 |
申请人 |
REG Life Sciences, LLC |
发明人 |
Hu Zhihao;Arlagadda Vikranth |
分类号 |
C12P7/02;C12P7/04;A61K38/00 |
主分类号 |
C12P7/02 |
代理机构 |
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代理人 |
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主权项 |
1. A method of producing a fatty alcohol composition comprising C12 and C14 fatty alcohols in a bacterial cell, the method comprising:
(a) expressing in the bacterial cell a gene encoding a polypeptide having thioesterase (EC 3.1.2- or EC 3.1.1.5) activity effective to release a fatty acid from acyl-ACP; and (b) further expressing in said bacterial cell an exogenous gene encoding a polypeptide having the amino acid sequence of SEQ ID NO:22; and (c) culturing said bacterial cell in culture media containing a carbohydrate carbon source under conditions wherein at least 25 mg of the composition is produced per liter of bacterial culture. |
地址 |
Ames IA US |