| 主权项 |
1. A method of determining whether a test sample contains Dengue virus, said method comprising the steps of:
(a) obtaining nucleic acids from the test sample; (b) performing an in vitro nucleic acid amplification reaction using the nucleic acids obtained in step (a) as templates for amplification with a set of primers, whereby there is produced an amplification product if said test sample comprised nucleic acids of any of Dengue virus serotypes 1-4, wherein the base sequence of a first member of said set of primers consists of a target-hybridizing sequence of SEQ ID NO:2, optionally joined to an upstream promoter sequence, and further optionally joined to an upstream tag sequence, wherein neither said upstream promoter sequence nor said upstream tag sequence can hybridize to the nucleic acid of any of Dengue virus serotypes 1-4 and participate in said in vitro nucleic acid amplification reaction in the absence of joining to said target-hybridizing sequence, and wherein the base sequence of a second member of said set of primers consists of SEQ ID NO:14; and (c) detecting, with a first detectably labeled hybridization probe, any of said amplification product that may have been produced in the in vitro nucleic acid amplification reaction, wherein the base sequence of said first detectably labeled hybridization probe is SEQ ID NO:22 or the complement thereof, allowing for substitution of RNA and DNA equivalent bases, whereby detecting said amplification product determines that the test sample contains at least one of Dengue virus serotypes 1-4, and whereby failing to detect said amplification product determines that the test sample does not contain Dengue virus. |
