Method for preparation of a biomolecule adsorbent

摘要

The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula:;
wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.

主权项

1. A method of preparing a biomolecule adsorbent with a pentadentate ligand of the formula wherein Q is a carrier S is a spacer L is an amide linkage X is COOH n =2 or 3 comprising the following steps:
providing an alkylene diamine tetraacetic acid dianhydride;providing an amino activated carrier;coupling said alkylene diamine tetraacetic acid dianhydride to said carrier to form ligands comprised of alkylene diamine triacetic acid linked to said carrier via an amide linkage and a spacer; andimmobilizing charged metal ion ions by charging the adsorbent so obtained with metal ions, selected from Cu2+, Ni2+, Zn2+, Co2+, Fe3+ and Ga3+ to form pentadentate ligands.